Large strain stimulation promotes extracellular matrix production and stiffness in an elastomeric scaffold model

Bioactive Extracellular Matrix Scaffold Promotes Adaptive Cardiac Remodeling and Repair

Effects of Biochemical and Mechanical Stimulation of Articular Chondrocytes in Collagen-GAG Scaffolds: Extracellular Matrix Biosynthesis and Scaffold Stiffness

As the incidence of osteoarthritis and other degenerative joint conditions continues to grow, rehabilitation via tissue engineering is becomingly increasingly attractive as an alternative to traditional surgical interventions. Chapters 2 and 3 of this thesis are specifically concerned with cartilage tissue engineering, while chapter 4 is relevant to bone and osteochondral tissue engineering. Th...

Reinforcement of esophageal anastomoses with an extracellular matrix scaffold in a canine model.

BACKGROUND The gastric pull-up procedure, a standard intervention after radical esophagectomy, is associated with high morbidity and mortality due to leaks and stricture. A previous preclinical study showed that an extracellular matrix (ECM) scaffold with autologous muscle tissue could be used to repair a complete circumferential defect in the cervical esophagus. The aim of the present study wa...

Extracellular matrix scaffold for cardiac repair.

BACKGROUND Heart failure remains a significant problem. Tissue-engineered cardiac patches offer potential to treat severe heart failure. We studied an extracellular matrix scaffold for repairing the infarcted left ventricle. METHODS AND RESULTS Pigs (n=42) underwent left ventricular (LV) infarction. At 6 to 8 weeks, either 4-layer multilaminate urinary bladder-derived extracellular matrix or ...

A porous PCL scaffold promotes the human chondrocytes redifferentiation and hyaline-specific extracellular matrix protein synthesis.

The redifferentiation, proliferation, and hyaline-specific extracellular matrix (ECM) protein synthesis of chondrocytes cultured in a polycaprolactone (PCL) scaffold were analyzed. Gene expression of the type II collagen and aggrecan was assessed by real-time PCR in cells from PCL scaffolds, monolayer, and pellet cultures. The proliferative activity was assessed using Ki-67 immunodetection, and...